UBC and YWHAZ as suitable reference genes for accurate normalisation of gene expression using MCF7, HCT116 and HepG2 cell lines

Relative quantification of in vitro gene expression using real-time PCR requires stably expressed reference gene for normalisation. In this study, total RNA from MCF7, HCT116 and HepG2 cells were extracted and converted to cDNA using commercially available kit, and real-time PCR was then performed t...

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Main Authors: Chua, Siang Ling, See Too, Wei Cun, Khoo, Boon Yin, Few, Ling Ling
格式: Artigo
語言:Inglês
出版: Springer Netherlands 2011
主題:
Original Research
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3217073/
https://ncbi.nlm.nih.gov/pubmed/21850463
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-011-9383-4
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