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Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control

BACKGROUND: The effects and effectiveness of the chaperone pair GroELS on the yield and quality of recombinant polypeptides produced in Escherichia coli are matter of controversy, as the reported activities of this complex are not always consistent and eventually indicate undesired side effects. The...

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Bibliografiske detaljer
Main Authors: Platas, Gemma, Rodríguez-Carmona, Escarlata, García-Fruitós, Elena, Cano-Garrido, Olivia, Villaverde, Antonio
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3207889/
https://ncbi.nlm.nih.gov/pubmed/21992454
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-79
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