Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control

BACKGROUND: The effects and effectiveness of the chaperone pair GroELS on the yield and quality of recombinant polypeptides produced in Escherichia coli are matter of controversy, as the reported activities of this complex are not always consistent and eventually indicate undesired side effects. The...

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Autores principales: Platas, Gemma, Rodríguez-Carmona, Escarlata, García-Fruitós, Elena, Cano-Garrido, Olivia, Villaverde, Antonio
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3207889/
https://ncbi.nlm.nih.gov/pubmed/21992454
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-79
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