Enzymatic replacement in vitro of the first anticodon base of yeast tRNAAsp: application to the study of tRNA maturation in vivo, after microinjection into frog oocytes.

A combination of several enzymes, RNase-T1, nuclease S1, T4-polynucleotide kinase and T4-RNA ligase were used to prepare and modify different fragments of yeast tRNAAsp (normal anticodon G U C). This allowed us to reconstitute, in vitro, a chimeric tRNA that has any of the four bases G, A, U or C, a...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Carbon, P, Haumont, E, De Henau, S, Keith, G, Grosjean, H
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1982
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320746/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6287419/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.12.3715
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