Enzymatic replacement in vitro of the first anticodon base of yeast tRNAAsp: application to the study of tRNA maturation in vivo, after microinjection into frog oocytes.

A combination of several enzymes, RNase-T1, nuclease S1, T4-polynucleotide kinase and T4-RNA ligase were used to prepare and modify different fragments of yeast tRNAAsp (normal anticodon G U C). This allowed us to reconstitute, in vitro, a chimeric tRNA that has any of the four bases G, A, U or C, a...

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Main Authors: Carbon, P, Haumont, E, De Henau, S, Keith, G, Grosjean, H
Formato: Artigo
Idioma:Inglês
Publicado: 1982
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC320746/
https://ncbi.nlm.nih.gov/pubmed/6287419
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