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Expression Screening of Fusion Partners from an E. coli Genome for Soluble Expression of Recombinant Proteins in a Cell-Free Protein Synthesis System

While access to soluble recombinant proteins is essential for a number of proteome studies, preparation of purified functional proteins is often limited by the protein solubility. In this study, potent solubility-enhancing fusion partners were screened from the repertoire of endogenous E. coli prote...

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Bibliografiske detaljer
Main Authors: Ahn, Jin-Ho, Keum, Jung-Won, Kim, Dong-Myung
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3206877/
https://ncbi.nlm.nih.gov/pubmed/22073212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0026875
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