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The preparative synthesis of oligodeoxyribonucleotides using RNA ligase.

The synthesis of nmol quantities of defined sequences of oligodeoxyribonucleotides using T4 RNA ligase has been demonstrated. Reacting using from 18 to 200 nmol of substrates in which a single 2'-deoxyribonucleoside 3',5'-bisphosphate was added to an oligodeoxyribonucleotide resulted in yields from...

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Udgivet i:Nucleic Acids Res
Principais autores: Hinton, D M, Brennan, C A, Gumport, R I
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1982
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320578/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7043396/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.6.1877
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