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STEADY-STATE AND TIME-RESOLVED FLUORESCENCE QUENCHING WITH TRANSITION METAL IONS AS SHORT-DISTANCE PROBES FOR PROTEIN CONFORMATION

A series of model dye-labeled histidine-containing peptides was used to investigate the nature of the quenching mechanism with Cu(2+) and Ni(2+). The strong reduction in steady-state fluorescence was found to be unaccompanied by any noticeable changes in lifetime kinetics. This static nature of quen...

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Detalhes bibliográficos
Main Authors: Posokhov, Yevgen O., Kyrychenko, Alexander, Ladokhin, Alexey S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3204958/
https://ncbi.nlm.nih.gov/pubmed/20707982
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.07.035
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