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RNAs synthesized using photocleavable biotinylated nucleotides have dramatically improved catalytic efficiency

Obtaining homogeneous population of natively folded RNAs is a crippling problem encountered when preparing RNAs for structural or enzymatic studies. Most of the traditional methods that are employed to prepare large quantities of RNAs involve procedures that partially denature the RNA. Here, we pres...

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Autors principals: Luo, Yiling, Eldho, Nadukkudy V., Sintim, Herman O., Dayie, T. Kwaku
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2011
Matèries:
RNA
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201860/
https://ncbi.nlm.nih.gov/pubmed/21742763
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr464
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