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RNAs synthesized using photocleavable biotinylated nucleotides have dramatically improved catalytic efficiency
Obtaining homogeneous population of natively folded RNAs is a crippling problem encountered when preparing RNAs for structural or enzymatic studies. Most of the traditional methods that are employed to prepare large quantities of RNAs involve procedures that partially denature the RNA. Here, we pres...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2011
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3201860/ https://ncbi.nlm.nih.gov/pubmed/21742763 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr464 |
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