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A Sensitive Alternative for MicroRNA In Situ Hybridizations Using Probes of 2′-O-Methyl RNA + LNA

The use of short, high-affinity probes consisting of a combination of DNA and locked nucleic acid (LNA) has enabled the specific detection of microRNAs (miRNAs) by in situ hybridization (ISH). However, detection of low–copy number miRNAs is still not always possible. Here the authors show that probe...

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Bibliographische Detailangaben
Hauptverfasser: Søe, Martin J., Møller, Trine, Dufva, Martin, Holmstrøm, Kim
Format: Artigo
Sprache:Inglês
Veröffentlicht: SAGE Publications 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201160/
https://ncbi.nlm.nih.gov/pubmed/21525189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155411409411
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