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A Sensitive Alternative for MicroRNA In Situ Hybridizations Using Probes of 2′-O-Methyl RNA + LNA

The use of short, high-affinity probes consisting of a combination of DNA and locked nucleic acid (LNA) has enabled the specific detection of microRNAs (miRNAs) by in situ hybridization (ISH). However, detection of low–copy number miRNAs is still not always possible. Here the authors show that probe...

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Detalhes bibliográficos
Main Authors: Søe, Martin J., Møller, Trine, Dufva, Martin, Holmstrøm, Kim
Formato: Artigo
Idioma:Inglês
Publicado em: SAGE Publications 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201160/
https://ncbi.nlm.nih.gov/pubmed/21525189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155411409411
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