Dramatically improved RNA in situ hybridization signals using LNA-modified probes

In situ detection of RNA by hybridization with complementary probes is a powerful technique. Probe design is a critical parameter in successful target detection. We have evaluated the efficiency of fluorescent DNA oligonucleotides modified to contain locked nucleic acid (LNA) residues. This increase...

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Main Authors: THOMSEN, RUNE, NIELSEN, PETER STEIN, JENSEN, TORBEN HEICK
Formato: Artigo
Idioma:Inglês
Publicado: Copyright 2005 by RNA Society 2005
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Method
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370861/
https://ncbi.nlm.nih.gov/pubmed/16177135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2139705
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