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OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons
Focused mutant library generation methods have been developed to improve mainly “localizable” enzyme properties such as activity and selectivity. Current multi-site saturation methods are restricted by the gene sequence, require subsequent PCR steps and/or additional enzymatic modifications. Here we...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3198389/ https://ncbi.nlm.nih.gov/pubmed/22039444 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0026222 |
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