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HPLC-Based Activity Profiling Approach for the Discovery of GABA(A) Receptor Ligands using an Automated Two Microelectrode Voltage Clamp Assay on Xenopus Oocytes

An approach for rapid HPLC-based profiling for new GABA(A) ligands of natural origin has been developed. Active extracts are separated by a single injection of 3 – 10 mg of extract onto a semi-preparative (150 × 10 mm i.d.) HPLC column with gradient elution and time-based fractionation. The microfra...

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Autors principals: Kim, Hyun Jung, Baburin, Igor, Khom, Sophia, Hering, Steffen, Hamburger, Matthias
Format: Artigo
Idioma:Inglês
Publicat: 2008
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3196969/
https://ncbi.nlm.nih.gov/pubmed/18543148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1055/s-2008-1074491
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