Cloning a cDNA for the pro-alpha 2 chain of human type I collagen.

Poly(A)-RNA enriched for type I procollagen sequences was isolated from normal human fibroblasts and used as template to synthesize double-stranded cDNA with avian myeloblastosis virus (AMV) reverse transcriptase. After the ends had been blunted with nuclease S1 and dGMP tails had been added with te...

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Bibliografiske detaljer
Main Authors: Myers, J C, Chu, M L, Faro, S H, Clark, W J, Prockop, D J, Ramirez, F
Format: Artigo
Sprog:Inglês
Udgivet: 1981
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC319600/
https://ncbi.nlm.nih.gov/pubmed/6267597
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