Cloning a cDNA for the pro-alpha 2 chain of human type I collagen.

Poly(A)-RNA enriched for type I procollagen sequences was isolated from normal human fibroblasts and used as template to synthesize double-stranded cDNA with avian myeloblastosis virus (AMV) reverse transcriptase. After the ends had been blunted with nuclease S1 and dGMP tails had been added with te...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Myers, J C, Chu, M L, Faro, S H, Clark, W J, Prockop, D J, Ramirez, F
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1981
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319600/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6267597/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.6.3516
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