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Cloning a cDNA for the pro-alpha 2 chain of human type I collagen.
Poly(A)-RNA enriched for type I procollagen sequences was isolated from normal human fibroblasts and used as template to synthesize double-stranded cDNA with avian myeloblastosis virus (AMV) reverse transcriptase. After the ends had been blunted with nuclease S1 and dGMP tails had been added with te...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1981
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319600/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6267597/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.6.3516 |
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