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Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.
Sub-genomic cDNA clones representing the entire genomic RNA of poliovirus Type 1 (Mahoney) have been isolated in E. coli. Construction of a complete cDNA copy of the poliovirus genome in the EcoRI site of plasmid vector pBR325 from these clones is described. Introduction of plasmid DNA containing th...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1984
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318905/ https://ncbi.nlm.nih.gov/pubmed/6330698 |
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