Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.
Sub-genomic cDNA clones representing the entire genomic RNA of poliovirus Type 1 (Mahoney) have been isolated in E. coli. Construction of a complete cDNA copy of the poliovirus genome in the EcoRI site of plasmid vector pBR325 from these clones is described. Introduction of plasmid DNA containing th...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1984
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318905/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330698/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.12.5123 |
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