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Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.

Sub-genomic cDNA clones representing the entire genomic RNA of poliovirus Type 1 (Mahoney) have been isolated in E. coli. Construction of a complete cDNA copy of the poliovirus genome in the EcoRI site of plasmid vector pBR325 from these clones is described. Introduction of plasmid DNA containing th...

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Bibliografiset tiedot
Julkaisussa:Nucleic Acids Res
Päätekijät: Semler, B L, Dorner, A J, Wimmer, E
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 1984
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318905/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330698/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.12.5123
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