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Point mutations in the middle of 16S ribosomal RNA of E. coli produced by deletion loop mutagenesis.
Using the plasmid pKK3535 , which contains the rrnB operon of Escherichia coli in pBR322, a deletion mutation was constructed which lacks bases 822 to 874 in the middle of the 16S ribosomal RNA. This results in an "amputation" of a very distinct stem and loop structure in the RNA. By formi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1984
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318837/ https://ncbi.nlm.nih.gov/pubmed/6328419 |
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