Point mutations in the middle of 16S ribosomal RNA of E. coli produced by deletion loop mutagenesis.
Using the plasmid pKK3535 , which contains the rrnB operon of Escherichia coli in pBR322, a deletion mutation was constructed which lacks bases 822 to 874 in the middle of the 16S ribosomal RNA. This results in an "amputation" of a very distinct stem and loop structure in the RNA. By forming a heter...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1984
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318837/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6328419/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.10.4361 |
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