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Point mutations in the middle of 16S ribosomal RNA of E. coli produced by deletion loop mutagenesis.

Using the plasmid pKK3535 , which contains the rrnB operon of Escherichia coli in pBR322, a deletion mutation was constructed which lacks bases 822 to 874 in the middle of the 16S ribosomal RNA. This results in an "amputation" of a very distinct stem and loop structure in the RNA. By formi...

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Bibliografiske detaljer
Main Authors: Zwieb, C, Dahlberg, A E
Format: Artigo
Sprog:Inglês
Udgivet: 1984
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC318837/
https://ncbi.nlm.nih.gov/pubmed/6328419
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