Construction and analysis of DNA sequence libraries from flow-sorted chromosomes: practical and theoretical considerations.

We describe the construction and analysis of recombinant DNA libraries representative of chromosomes 1 and 2 of Chinese hamster (Cricetulus griseus). Propidium-iodide stained chromosomes were purified by flow cytometric analysis and sorting, and EcoRI digests of purified DNA were cloned into the bac...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Griffith, J K, Cram, L S, Crawford, B D, Jackson, P J, Schilling, J, Schimke, R T, Walters, R A, Wilder, M E, Jett, J H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1984
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318808/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6587323/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.9.4019
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