Construction and analysis of DNA sequence libraries from flow-sorted chromosomes: practical and theoretical considerations.
We describe the construction and analysis of recombinant DNA libraries representative of chromosomes 1 and 2 of Chinese hamster (Cricetulus griseus). Propidium-iodide stained chromosomes were purified by flow cytometric analysis and sorting, and EcoRI digests of purified DNA were cloned into the bac...
Tallennettuna:
| Julkaisussa: | Nucleic Acids Res |
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| Päätekijät: | , , , , , , , , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
Oxford University Press
1984
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| Linkit: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318808/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6587323/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.9.4019 |
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