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Construction and analysis of DNA sequence libraries from flow-sorted chromosomes: practical and theoretical considerations.
We describe the construction and analysis of recombinant DNA libraries representative of chromosomes 1 and 2 of Chinese hamster (Cricetulus griseus). Propidium-iodide stained chromosomes were purified by flow cytometric analysis and sorting, and EcoRI digests of purified DNA were cloned into the bac...
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| Main Authors: | , , , , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1984
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318808/ https://ncbi.nlm.nih.gov/pubmed/6587323 |
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