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Construction and analysis of DNA sequence libraries from flow-sorted chromosomes: practical and theoretical considerations.

We describe the construction and analysis of recombinant DNA libraries representative of chromosomes 1 and 2 of Chinese hamster (Cricetulus griseus). Propidium-iodide stained chromosomes were purified by flow cytometric analysis and sorting, and EcoRI digests of purified DNA were cloned into the bac...

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Detalles Bibliográficos
Main Authors: Griffith, J K, Cram, L S, Crawford, B D, Jackson, P J, Schilling, J, Schimke, R T, Walters, R A, Wilder, M E, Jett, J H
Formato: Artigo
Idioma:Inglês
Publicado: 1984
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC318808/
https://ncbi.nlm.nih.gov/pubmed/6587323
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