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Minibodies and Multimodal Chromatography Methods: A Convergence of Challenge and Opportunity

This case study describes early phase purification process development for a recombinant anticancer minibody produced in mammalian cell culture. The minibody did not bind to protein A. Cation-exchange, anion-exchange, hydrophobic-interaction, and hydroxyapatite (eluted by phosphate gradient) chromat...

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Detalhes bibliográficos
Main Authors: Gagnon, Pete, Cheung, Chia-Wei, Lepin, Eric J., Wu, Anna M., Sherman, Mark A., Raubitschek, Andrew A., Yazaki, Paul J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3187938/
https://ncbi.nlm.nih.gov/pubmed/21984873
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