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Reverse calcium affinity purification of Fab with calcium derivatized hydroxyapatite
This study introduces the application of calcium-derivatized hydroxyapatite for purification of Fab. Fab binds to native hydroxyapatite but fails to bind to the calcium derivatized form. IgG, Fc, and most other protein contaminants bind to the calcium form. This supports Fab purification by a simple...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3171993/ https://ncbi.nlm.nih.gov/pubmed/19150352 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2008.11.021 |
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