Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.

3'-O-(5'-phosphoryldeoxycytidyl) phosphorothioate and fluorescent 3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate can be ligated to tRNA by T4 RNA ligase. They are also efficient donors for the enzymatic ligation to oligodeoxynucleotides bearing a 3'-cytidine terminus. Cytidine 3',5'-bisp...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Cosstick, R, McLaughlin, L W, Eckstein, F
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1984
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318621/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6366740/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.4.1791
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