Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.
3'-O-(5'-phosphoryldeoxycytidyl) phosphorothioate and fluorescent 3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate can be ligated to tRNA by T4 RNA ligase. They are also efficient donors for the enzymatic ligation to oligodeoxynucleotides bearing a 3'-cytidine terminus. Cytidine 3',5'-bisp...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1984
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318621/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6366740/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.4.1791 |
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