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Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.

3'-O-(5'-phosphoryldeoxycytidyl) phosphorothioate and fluorescent 3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate can be ligated to tRNA by T4 RNA ligase. They are also efficient donors for the enzymatic ligation to oligodeoxynucleotides bearing a 3'-cytidine term...

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Bibliografiske detaljer
Main Authors: Cosstick, R, McLaughlin, L W, Eckstein, F
Format: Artigo
Sprog:Inglês
Udgivet: 1984
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC318621/
https://ncbi.nlm.nih.gov/pubmed/6366740
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