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Accessing protein conformational ensembles using room-temperature X-ray crystallography

Modern protein crystal structures are based nearly exclusively on X-ray data collected at cryogenic temperatures (generally 100 K). The cooling process is thought to introduce little bias in the functional interpretation of structural results, because cryogenic temperatures minimally perturb the ove...

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Detalhes bibliográficos
Main Authors: Fraser, James S., van den Bedem, Henry, Samelson, Avi J., Lang, P. Therese, Holton, James M., Echols, Nathaniel, Alber, Tom
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3182744/
https://ncbi.nlm.nih.gov/pubmed/21918110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1111325108
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