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Accessing protein conformational ensembles using room-temperature X-ray crystallography

Modern protein crystal structures are based nearly exclusively on X-ray data collected at cryogenic temperatures (generally 100 K). The cooling process is thought to introduce little bias in the functional interpretation of structural results, because cryogenic temperatures minimally perturb the ove...

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Autors principals: Fraser, James S., van den Bedem, Henry, Samelson, Avi J., Lang, P. Therese, Holton, James M., Echols, Nathaniel, Alber, Tom
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3182744/
https://ncbi.nlm.nih.gov/pubmed/21918110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1111325108
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