Labelling oligonucleotides to high specific activity (I).
The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and gamma 32P-ATP. However, this has the disadvantage of only introducing one labelled base per molecule of the oligonucleotide. In this paper we describe an approach based on primer/template combin...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1989
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318018/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2526319/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.12.4605 |
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