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Labelling oligonucleotides to high specific activity (I).

The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and gamma 32P-ATP. However, this has the disadvantage of only introducing one labelled base per molecule of the oligonucleotide. In this paper we describe an approach based on primer/template combin...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Craig, A G, Nizetic, D, Lehrach, H
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1989
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318018/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2526319/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.12.4605
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