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Labelling oligonucleotides to high specific activity (I).
The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and gamma 32P-ATP. However, this has the disadvantage of only introducing one labelled base per molecule of the oligonucleotide. In this paper we describe an approach based on primer/template combin...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1989
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318018/ https://ncbi.nlm.nih.gov/pubmed/2526319 |
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