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Enzymatic synthesis of long double-stranded DNA labeled with haloderivatives of nucleobases in a precisely pre-determined sequence

BACKGROUND: Restriction endonucleases are widely applied in recombinant DNA technology. Among them, enzymes of class IIS, which cleave DNA beyond recognition sites, are especially useful. We use BsaI enzyme for the pinpoint introduction of halogen nucleobases into DNA. This has been done for the pur...

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書誌詳細
主要な著者: Sobolewski, Ireneusz, Polska, Katarzyna, Żylicz-Stachula, Agnieszka, Jeżewska-Frąckowiak, Joanna, Rak, Janusz, Skowron, Piotr
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2011
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3179937/
https://ncbi.nlm.nih.gov/pubmed/21864341
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-12-47
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