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Novel parameter describing restriction endonucleases: Secondary-Cognate-Specificity and chemical stimulation of TsoI leading to substrate specificity change

Over 470 prototype Type II restriction endonucleases (REases) are currently known. Most recognise specific DNA sequences 4–8 bp long, with very few exceptions cleaving DNA more frequently. TsoI is a thermostable Type IIC enzyme that recognises the DNA sequence TARCCA (R = A or G) and cleaves downstr...

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Detalhes bibliográficos
Publicado no:Appl Microbiol Biotechnol
Main Authors: Zebrowska, Joanna, Jezewska-Frackowiak, Joanna, Wieczerzak, Ewa, Kasprzykowski, Franciszek, Zylicz-Stachula, Agnieszka, Skowron, Piotr M.
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6449304/
https://ncbi.nlm.nih.gov/pubmed/30879089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-019-09731-0
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