Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

BACKGROUND: In continuing our research into the new family of bifunctional restriction endonucleases (REases), we describe the cloning of the tsoIRM gene. Currently, the family includes six thermostable enzymes: TaqII, Tth111II, TthHB27I, TspGWI, TspDTI, TsoI, isolated from various Thermus sp. and t...

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Auteurs principaux: Skowron, Piotr M, Vitkute, Jolanta, Ramanauskaite, Danute, Mitkaite, Goda, Jezewska-Frackowiak, Joanna, Zebrowska, Joanna, Zylicz-Stachula, Agnieszka, Lubys, Arvydas
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2013
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3751577/
https://ncbi.nlm.nih.gov/pubmed/23919831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-14-17
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