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Enzymatic synthesis of long double-stranded DNA labeled with haloderivatives of nucleobases in a precisely pre-determined sequence

BACKGROUND: Restriction endonucleases are widely applied in recombinant DNA technology. Among them, enzymes of class IIS, which cleave DNA beyond recognition sites, are especially useful. We use BsaI enzyme for the pinpoint introduction of halogen nucleobases into DNA. This has been done for the pur...

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Detalhes bibliográficos
Main Authors: Sobolewski, Ireneusz, Polska, Katarzyna, Żylicz-Stachula, Agnieszka, Jeżewska-Frąckowiak, Joanna, Rak, Janusz, Skowron, Piotr
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3179937/
https://ncbi.nlm.nih.gov/pubmed/21864341
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-12-47
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