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A method for introducing random single point deletions in specific DNA target sequences using oligonucleotides.

We describe a method for the generation of random point deletions in any target DNA sequence using synthetic mixed oligonucleotides. A mixed pool of oligonucleotides, which contain single nucleotide deletions randomly distributed throughout the full length, was generated by a modification of the syn...

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Sonraí Bibleagrafaíochta
Main Authors: Ner, S S, Atkinson, T C, Smith, M
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1989
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC317915/
https://ncbi.nlm.nih.gov/pubmed/2662137
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