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Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.
A method for constructing yeast artificial chromosome (YAC) libraries with large insert sizes is reported. High molecular weight human DNA was partially digested with EcoRI and cloned in the vector pYAC4. When unfractionated DNA was used, the mean YAC size was 120kb. Fractionation by pulsed-field ge...
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| Main Authors: | , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1989
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC317785/ https://ncbi.nlm.nih.gov/pubmed/2542900 |
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