Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.
A method for constructing yeast artificial chromosome (YAC) libraries with large insert sizes is reported. High molecular weight human DNA was partially digested with EcoRI and cloned in the vector pYAC4. When unfractionated DNA was used, the mean YAC size was 120kb. Fractionation by pulsed-field ge...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1989
|
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317785/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2542900/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.9.3425 |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|