Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.
A method for constructing yeast artificial chromosome (YAC) libraries with large insert sizes is reported. High molecular weight human DNA was partially digested with EcoRI and cloned in the vector pYAC4. When unfractionated DNA was used, the mean YAC size was 120kb. Fractionation by pulsed-field ge...
保存先:
| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1989
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317785/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2542900/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.9.3425 |
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