PCR-based cDNA library construction: general cDNA libraries at the level of a few cells.
A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers c...
Kaydedildi:
| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1989
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| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317702/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2471144/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.8.2919 |
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