PCR-based cDNA library construction: general cDNA libraries at the level of a few cells.
A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers c...
-д хадгалсан:
| -д хэвлэсэн: | Nucleic Acids Res |
|---|---|
| Үндсэн зохиолчид: | , , |
| Формат: | Artigo |
| Хэл сонгох: | Inglês |
| Хэвлэсэн: |
Oxford University Press
1989
|
| Онлайн хандалт: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317702/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2471144/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.8.2919 |
| Шошгууд: |
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
|