PCR-based cDNA library construction: general cDNA libraries at the level of a few cells.
A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers c...
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| 發表在: | Nucleic Acids Res |
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| Principais autores: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
1989
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| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317702/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2471144/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.8.2919 |
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