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Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples
BACKGROUND: Real-time PCR is a sensitive and specific method for the analysis of Plasmodium DNA. However, prior purification of genomic DNA from blood is necessary since PCR inhibitors and quenching of fluorophores from blood prevent efficient amplification and detection of PCR products. METHODS: Re...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3171379/ https://ncbi.nlm.nih.gov/pubmed/21851640 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2875-10-244 |
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