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Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples

BACKGROUND: Real-time PCR is a sensitive and specific method for the analysis of Plasmodium DNA. However, prior purification of genomic DNA from blood is necessary since PCR inhibitors and quenching of fluorophores from blood prevent efficient amplification and detection of PCR products. METHODS: Re...

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書誌詳細
主要な著者: Taylor, Brian J, Martin, Kimberly A, Arango, Eliana, Agudelo, Olga M, Maestre, Amanda, Yanow, Stephanie K
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2011
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3171379/
https://ncbi.nlm.nih.gov/pubmed/21851640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2875-10-244
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