Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples

BACKGROUND: Real-time PCR is a sensitive and specific method for the analysis of Plasmodium DNA. However, prior purification of genomic DNA from blood is necessary since PCR inhibitors and quenching of fluorophores from blood prevent efficient amplification and detection of PCR products. METHODS: Re...

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Bibliographische Detailangaben
Hauptverfasser: Taylor, Brian J, Martin, Kimberly A, Arango, Eliana, Agudelo, Olga M, Maestre, Amanda, Yanow, Stephanie K
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2011
Schlagworte:
Methodology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3171379/
https://ncbi.nlm.nih.gov/pubmed/21851640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2875-10-244
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