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Mapping protein interactions by combining antibody affinity maturation and mass spectrometry

Mapping protein interactions by immunoprecipitation is limited by the availability of antibodies recognizing available native epitopes within protein complexes with sufficient affinity. Here we demonstrate a scalable approach for generation of such antibodies using phage display and affinity maturat...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Dyson, Michael R., Zheng, Yong, Zhang, Cunjie, Colwill, Karen, Pershad, Kritika, Kay, Brian K., Pawson, Tony, McCafferty, John
Format: Artigo
Sprache:Inglês
Veröffentlicht: Academic Press 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3171153/
https://ncbi.nlm.nih.gov/pubmed/21704603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.05.005
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