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Mapping protein interactions by combining antibody affinity maturation and mass spectrometry

Mapping protein interactions by immunoprecipitation is limited by the availability of antibodies recognizing available native epitopes within protein complexes with sufficient affinity. Here we demonstrate a scalable approach for generation of such antibodies using phage display and affinity maturat...

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Main Authors: Dyson, Michael R., Zheng, Yong, Zhang, Cunjie, Colwill, Karen, Pershad, Kritika, Kay, Brian K., Pawson, Tony, McCafferty, John
格式: Artigo
語言:Inglês
出版: Academic Press 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3171153/
https://ncbi.nlm.nih.gov/pubmed/21704603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.05.005
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