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Mapping protein interactions by combining antibody affinity maturation and mass spectrometry

Mapping protein interactions by immunoprecipitation is limited by the availability of antibodies recognizing available native epitopes within protein complexes with sufficient affinity. Here we demonstrate a scalable approach for generation of such antibodies using phage display and affinity maturat...

詳細記述

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書誌詳細
主要な著者: Dyson, Michael R., Zheng, Yong, Zhang, Cunjie, Colwill, Karen, Pershad, Kritika, Kay, Brian K., Pawson, Tony, McCafferty, John
フォーマット: Artigo
言語:Inglês
出版事項: Academic Press 2011
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3171153/
https://ncbi.nlm.nih.gov/pubmed/21704603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.05.005
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