Preferential relaxation of positively supercoiled DNA by E. coli topoisomerase IV in single-molecule and ensemble measurements

We show that positively supercoiled [(+) SC] DNA is the preferred substrate for Escherichia coli topoisomerase IV (topo IV). We measured topo IV relaxation of (−) and (+) supercoils in real time on single, tethered DNA molecules to complement ensemble experiments. We find that the preference for (+)...

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Detalhes bibliográficos

Publicado no:	Genes Dev
Principais autores:	Crisona, Nancy J., Strick, Terence R., Bensimon, David, Croquette, Vincent, Cozzarelli, Nicholas R.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Cold Spring Harbor Laboratory Press 2000
Assuntos:	Research Paper
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317058/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11090135/ https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gad.838900
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